Sanguis SuperDirectᵀᴹ PCR Kit(ung) -Heparin Sanguis Direct PCR Master Mix pro Genotyping Sanguinis
Ornamentum Description:
Directe utere heparin anticoagulato integro sanguine sicut exempli causa amplificationis PCR, cum summa sensibilitate et amplificationis magnae efficientiae.Systema anti-pollutionis UNG ad positivis falsis praecavendis addita est.
◮Systema amplificandi facultatem validam habet ac sensim potest detegere genome et aliena scopum DNA fragmenta in sanguine.
◮2× SuperEasyTMMix(ung) -Heparin Maximum sanguinem tolerantia: usque ad 45% in sanguine humano et 20% in sanguine muris.
◮Specimen plene inclusum est, quare solliciti esse non debes de sample contagione et falsis positivis PCR proventuum.Anti-pollutio PCR systema
◮2×SuperEasyTMMisce (ung), pollutio quae per PCR productorum causata efficaciter eliminat, et amplificationis proprietatem et accurationem curet.
Specifications
200 ×20μl rxns, 2000 ×20μl rxns
2× SuperEasyTMMiscere (ung) -Heparin, ex II "SuperEasy"TMMix-Heparin, dUTP loco dTTP adhibetur, et enzyme UNG, quod dUTP templates degradare potest, simul additur, quae efficaciter impedire potest contagione PCR amplificationis productorum.Polymerase, dNTPs, MgCl2, D-Taq DNA specialiter modificatum continet, reactionem quiddam, PCR reactionem augentem, optimizer et stabilientem, etc. In PCR reactionem, modo congruentem totum sanguinem, primers et ddH anticoagulatum addas.2O ad respondentem 2× SuperEasyTM Mix(UNG)-Heparin adhibendum in pcr reactionem.
6× DNA Loading Buffer: Loading Buffer non continet SDS.Commendatur uti 6× DNA Loading Buffer inclusa cum ornamento agaroso gel electronico incluso ad consequendum bonum electronicophoresis proventum.Noli uti Buffer loading continens SDS, alioquin magna cauda lucis lucidae in angiporto erit in electrophoresi, quae experimentalem eventum afficiet.
Ornamentum components
| 2× SuperEasyTMMix(ung)-Heparin |
| VI "DNA Loading Buffer" |
| Instructiones |
Features & commoda
-Systema amplificandi facultatem validam habet ac sensim potest detegere genome et aliena scopum DNA fragmenta in sanguine.
-2× SuperEasyTMMix(ung) -Heparin Maximum sanguinem tolerantia: usque ad 45% in sanguine humano et 20% in sanguine muris.
- Exemplar plene inclusum est, quare solliciti esse non debes de sample contagione et falsis positivis PCR proventuum.Anti-pollutio PCR ratio 2×SuperEasyTMMisce (ung), pollutio quae per PCR productorum causata efficaciter eliminat, et amplificationis proprietatem et accurationem curet.
Ornamentum application
PCR identificatio de Heparin anticoagulato totius sanguinis directa dicata.(including: humanus sanguis, mus sanguis, pullus sanguis, avis sanguis, bos sanguis, canis sanguis, etc.)
Workflow
Repono et fasciae vitae
Totum processum capsulae glaciei humilis temperaturae vecturae est, ut ornamentum sit in statu <4 ℃.
2× SuperEasyTMMisce(ung)-Heparin, si saepius usus est, etiam reponi potest ad 4°C ad breve tempus repositione (utendum est intra 10 dies).
6× DNA Buffer Loading, 4°C vel -20°C diu condi potest.
