Direct RT-qPCR Kit
Ornamentum Description:
◮ Direct RT-qPCR Kit, utens Foreasy Reverse Transscriptas et Foreasy HS Taq DNA Polymerase per Foregene explicans, cum unico quiddam reactionis, hoc ornamentum cum fortiter resistendo et compatibilitate facit, et potest directe test reactionem uti Systema Foregene Lysis pro template.Hoc ornamentum deprehensio acidi nucleici applicare potest ad COVID-19 ornamentum et alia bacteria nuclei morbi detectionis ornamentum acidi evolutionis.
Descriptio
Directum RT-qPCR Kit separatim packaged RT-qPCR quiddam praebet et altum enzyme efficientemixtura (Taq、M-MLV、RI) faciens opportunum ad partum tergum finem rhoncus et ratiotemperatio-in simplex verificationem.
Specifications
500T, 50,000T .
Ornamentum components
| Ornamentum contenta (20 μL ratio reactionis) | IM-05111-01 | IM-05112-01 |
| 500 T | 50,000 T | |
| Direct Enzyme Mixture | 500 μL1 | 50 mL1 |
| 2× Direct RT-qPCR Buffer | 1 mL5 | 500 mL1 |
| Disciplinam | 1 | 1 |
Gradus operating
Ante praeparationem reagens, omnes regentes in ornamentum in cella temperies divelli debent et suaviter misceri.
A: para of template et tenui
1. Praeparata RNA templates (commendatur ad usum Foregenae Totalis RNA Isolation Kit series ornamentum extrahere et purgare RNA template) vel specimen productum crepuisse (commendatur ut systema Foregene Lysis), certis primers(10 μM) et alia quae pertinentes consumabiles, instrumenti.
2. Pone 2× Direct RT-qPCR Buffer, RNase-Free ddH2O et 20× ROX Reference Dye (si'opus s) in pyxide glaciali, quae naturaliter liquefiunt, ac leniter mixta.
B: para of RT-qPCR systema
Mensa 1 : para of RT-qPCR system
| Component | Magnitudo | Finalis concentration |
| 2× Direct RT-qPCR Buffer | 10 μL | 1× |
| Direct Enzyme Mixture | 1μL | |
| Deinceps primario(10 μM) | 0.8 μL | 50-900nM |
| Inversa primario(10 μM) | 0.8 μL | 50-900nM |
| Probe (4 μM) | 1μL | 200nM |
| Formula(RNA vel Lysate) | X μL | |
| 20×ROX Reference Dye | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Totalis Volume | 20μL |
C: Set the RT-qPCR reactionem Program
Mensa 2 : RT-qPCR reaction Program occasum (for* exemplum: duo-step methodo)
| Gradus | Temperature | Tempus | Cycles | Content | |
| 1 | 50℃ | 15 min | 1* | 1 | Reverse transcription |
| 2 | 95℃ | 1 min | 1 | Pre-denaturation | |
| 3 | 95℃ | 10 sec | 40-45 | Denaturation | |
| 60℃ | 30 sec 2* | Anneal/Extensio | |||
Repono et fasciae vitae
Id ornamentum condi debet -20± 5℃.Validitas periodus 12 mensium est.
-Age saepe duratus CALEFACTO cyclos (<5 circuitus).
