ForeDirect RT-qPCR Kit
Ornamentum Description:
◮Pro RNA amplificatio reali temporis directa e collectionibus swab sine RNA processuum purificationis est.Hoc ornamentum qRT-PER cyclos intra unam horam complet.Miscere enzyme est mixtio optimized inversa Transscriptae, Hot-Start Taq DNA polymerase, RNase inhibitor.Reactio Buffer continet omnes partes requisitarum, inclusas quiddam optimized partium, Mg2+dUTP, et dNTPs.
Descriptio
ForeDirect RT-qPCR Ornamentum ordinatur ad RNA amplificationem realem realem temporis directae collectiones swab sine RNA processuum purificationis.Hoc ornamentum qRT-PER cyclos intra unam horam complet.Miscere enzyme est mixtio optimized inversa Transscriptae, Hot-Start Taq DNA polymerase, RNase inhibitor.Reactio Buffer continet omnes partes requisitarum, inclusas quiddam optimized partium, Mg2+dUTP, et dNTPs.
Specifications
100T
Ornamentum components
| Acidum nucleicum emissio agentis |
| RNA protector |
| 2× RT-qPCR Buffer |
| Enzyme Mixture (Taq&M-MLV) |
| Instructiones |
PCR Application
1. Postquam per reactionem fistulae breviter centrifugae factae sunt, eas in sample piscina pone instrumenti amplificationis.
2. Scelerisque protocol
| Gradus | Temperature | Tempus | Cycles | |
| 1 | Reverse transcription | 50℃ | 15 min | 1 |
| 2 | Pre-denaturation | 95℃ | 1 min | 1 |
| 3 | Denaturation | 95℃ | X seconds | 42 |
| 4 | Anneal/Extensio | 60℃ | XXX seconds | |
Nota:Signum Fluorescentis deprehensum est statim post extensionem gradus cuiusque cycli.
3. Post occasum, lima serva et curriculum reactionis.
Gradus operating
Ante praeparationem reagens, omnes regentes in ornamentum in cella temperies divelli debent et suaviter misceri.
Praeparatio reagens
1. Para nuclei Acidum Release Mix
| Component | Volume per Sample seu Imperium |
| Acidum nucleicum emissio agentis | 5μL |
| RNA protector | 0.5 μL |
2. Para reactionem Mix
| Component | Volume per Sample seu Imperium |
| 2× RT-qPCR Buffer | 15 μL |
| Enzyme Mixture (Taq&M-MLV) | 1.5 μL |
| Primi et Probes | 1μL |
Repono et fasciae vitae
Id ornamentum condi debet -20± 5℃.Validitas periodus 12 mensium est.
-Age saepe duratus CALEFACTO cyclos (<5 circuitus).
